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A repressible operon is usually controlled by:


A) an inactive repressor that allows it to be in the "on" state.
B) the supply of the precursor product for the enzymes.
C) an inactive repressor that keeps it in the "off" state.
D) tryptophan.
E) being turned "on," usually by the end product of the pathway.

F) C) and E)
G) All of the above

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Temporal gene regulation refers to which of the following circumstances?


A) Certain genes are induced only at a particular point in the life cycle.
B) Certain genes are induced only in specific tissues.
C) Heat-shock genes are induced during periods of temperature stress.
D) "Housekeeping" genes are induced in response to a viral infection.
E) Molecular chaperones are synthesized after heavy-metal ingestion.

F) A) and B)
G) None of the above

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In the tryptophan operon, the repressor actively binds to the operator when:


A) tryptophan binds to an allosteric site on the repressor.
B) tryptophan levels are low.
C) lactose levels are low.
D) there is a great need for proteins.
E) allotryptophan levels are low.

F) A) and E)
G) B) and C)

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DNA sequences called __________ increase the rate of RNA synthesis after initiation of transcription.


A) promoters
B) TATA boxes
C) UPEs
D) enhancers
E) regulators

F) D) and E)
G) A) and D)

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Explain the respective advantages associated with operons being inducible (e.g., the lactose operon) or repressible (e.g., the tryptophan operon).

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Inducible operons save energy for the prokaryotes because the proteins of the operon are only synthesized when needed for survival. The advantage of a repressible operons is that they prevent excess production of a metabolite.

How does the lactose repressor block transcription of the lactose operon?


A) by "turning off" the appropriate genes in the intron
B) by regulating the activity of the enzymes that the operon codes for
C) by binding allosterically to the appropriate genes
D) by slowing the uptake of lactose into the cell
E) by binding to the operator

F) None of the above
G) D) and E)

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Regulation of gene expression can NOT be accomplished by controlling:


A) the activity of a protein product.
B) the amount of mRNA that is available.
C) the rate of translation of mRNA.
D) the rate of mRNA degradation.
E) the rate of DNA replication

F) A) and E)
G) A) and D)

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A repressor operon regulates the gene expression of anabolic pathways. __________________

A) True
B) False

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True

Some eukaryotic DNA sequences act as introns in the cells of some tissues and exons in the cells of other tissues.This allows:


A) prevention of harmful mutation.
B) regulation of the rate of transcription.
C) formation of different types of closely related proteins.
D) regulation of the rate of translation.
E) repression of DNA replication.

F) C) and D)
G) A) and B)

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MATCHING Match the type of gene regulation with the process. a.regulation of transcription d.regulation of translation b.regulation of mRNA processing e.regulation of chromatin c.regulation of mRNA transport -Controlling access to nuclear pores

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Mutations in the TATA box increase the rate of transcription. __________________

A) True
B) False

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Genes in euchromatic regions are:


A) inhibited.
B) duplicated
C) actively being transcribed.
D) repressed.
E) unregulated.

F) A) and E)
G) B) and D)

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MATCHING Match the type of gene regulation with the process. a.regulation of transcription d.regulation of translation b.regulation of mRNA processing e.regulation of chromatin c.regulation of mRNA transport -Unpacked heterochromatin

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Feedback inhibition is:


A) transcriptional control.
B) transcriptional control
C) a mechanism affecting events after translation.
D) a repressible system.
E) constitiutive control

F) A) and B)
G) B) and D)

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The operator of the lactose operon in E.coli is located:


A) between the promoter and the TATA box.
B) upstream of the promoter.
C) between the promoter and the structural genes.
D) among the structural genes.
E) anywhere on the chromosome.

F) B) and C)
G) A) and E)

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C

An inducible operon is usually controlled by:


A) an inducer molecule that keeps it in the "off" state.
B) an active repressor that keeps it in the "off" state.
C) being active at all times.
D) allolactose.
E) being turned "off," usually by the end product of the pathway.

F) B) and E)
G) A) and D)

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A cell may meet the need for large quantities of a specific protein by all of the following EXCEPT :


A) continuously synthesizing the mRNA molecule that specifies that protein.
B) increasing the half-life of the mRNA that specifies the protein.
C) having multiple copies of the gene that codes for that protein.
D) amplifying protein genes by DNA replication
E) inhibition of DNA synthesis

F) A) and D)
G) A) and E)

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Why is it important that a few β\beta -galactosidase molecules be present in E.coli even if these cells have been growing on a lactose-free medium?

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Concepts to Consider: It is im...

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The gene that codes for the repressor protein of the E.coli lactose operon is:


A) located between the operator and the promoter.
B) downstream from the promoter region.
C) downstream from the operator.
D) constitutive.
E) turned off most of the time.

F) A) and C)
G) C) and D)

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In female mammals, most of the inactive X chromosome has become:


A) associated with histones.
B) active X factor.
C) euchromatin.
D) replicated.
E) heterochromatin.

F) All of the above
G) A) and B)

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