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The Six I's of studying microorganisms include all of the following except


A) inoculation.
B) incubation.
C) infection.
D) isolation.
E) identification.

F) A) and D)
G) A) and C)

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Compare and contrast the reagents and functions of negative staining versus positive staining.

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Negative staining and positive staining ...

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A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?


A) The microbiologist used too much inoculum.
B) The culture is contaminated.
C) The incubation temperature was incorrect.
D) The culture medium must be selective.
E) The culture medium must be differential.

F) A) and E)
G) C) and D)

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Mannitol salt agar is selective for which bacterial genus?


A) Salmonella
B) Streptococcus
C) Neisseria
D) Staphylococcus
E) Escherichia

F) C) and E)
G) A) and E)

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All of the following pertain to the fluorescence microscope except it


A) uses electron's to produce a specimen image.
B) is a type of compound microscope.
C) requires the use of dyes like acridine and fluorescein.
D) is commonly used to diagnose certain infections.
E) requires an ultraviolet radiation source.

F) A) and B)
G) A) and C)

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All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except


A) 0.2 µm.
B) 0.2 mm.
C) 0.1 µm.
D) 0.3 µm.
E) 2.0 µm.

F) A) and B)
G) A) and E)

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Mixed cultures are also referred to as contaminated cultures.

A) True
B) False

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Why is immersion oil often used when viewing specimens under the microscope?


A) to stain the cells blue
B) to prevent the smear from drying out
C) to slow bacterial movement so you can see cells better
D) to increase the resolution
E) to reduce the amount of heat reaching the slide from the light source

F) A) and C)
G) All of the above

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In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?


A) Sterile swab, loop dilution, TSB
B) Hockey stick, spread plate technique, and 4 degree Celsius incubation temperature
C) Loop dilution, TSA, hockey stick, 37 degree Celsius incubator
D) Inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator
E) Sterile swab, streak plate method, 37 degree Celsius incubator

F) B) and C)
G) B) and D)

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Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

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One colony typically develops from the growth of several parent bacterial cells.

A) True
B) False

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A contaminated food sample contains several different species of bacteria.A food microbiologist is interested in studying just one of these species.Describe the sequence of procedures that the microbiologist must perform in order to obtain a pure culture of the bacterial species of interest from this food sample.Detail all the necessary media and equipment.

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The microbiologist must first obtain a s...

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The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the _____.

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The Gram stain, acid-fast stain, and endospore stain ____________.


A) are used on a wet mount of the specimen
B) use heat to force the dye into cell structures
C) have outcomes based on cell wall differences
D) use a negative stain technique
E) are differential stains

F) B) and C)
G) A) and B)

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Sally had a throat sample taken at a satellite lab within her health care provider's office.What kind of media would be best suited for this specimen?


A) TSA plate
B) TSB tube
C) Transport media
D) Pour plate
E) Quadrant streak plate

F) C) and E)
G) A) and D)

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C

_____ is the term for a culture made from one isolated colony.

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Bacteria that require special growth factors and complex nutrients are termed _____.

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Identification relies entirely on biochemical test results.

A) True
B) False

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False

The type of microscope in which you would see brightly illuminated specimens against a black background is


A) bright-field.
B) dark-field.
C) phase-contrast.
D) fluorescence.
E) electron.

F) All of the above
G) A) and B)

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B

Media that contains extracts from plants, animals, or yeasts are


A) synthetic.
B) complex.
C) reducing.
D) enriched.
E) All of the choices are correct.

F) A) and C)
G) A) and E)

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