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During the process of gene expression, RNA polymerase would most likely be found


A) in a highly repetitive sequence.
B) in a noncoding sequence.
C) in an open reading frame.
D) at a metagenomic site.
E) in a pseudogene.

F) None of the above
G) A) and B)

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Which of the following is not a main type of transposon in the human genome?


A) SINEs
B) LINEs
C) DNA transposons
D) Retrotransposons
E) Each of the above is a main type of human transposon.

F) A) and C)
G) A) and D)

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The type of sequencing known as _______ relies on sophisticated computer algorithms to align numerous short DNA sequences without the use of a genetic map.

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A study comparing the proteins that are produced in sperm cells to those of skin cells would be an example of


A) comparative genomics.
B) functional genomics.
C) pharmacogenomics.
D) metabolomics.
E) proteomics.

F) B) and D)
G) A) and B)

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The maximum length of DNA that can be sequenced at one time by means of the standard technology is about _______ bases.


A) 700
B) 3,000
C) 7,000
D) 30,000
E) 70,000

F) None of the above
G) A) and B)

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The coding region of a gene, also known as a(n) _______, can usually be recognized by the presence of a(n) _______ codon and a(n) _______ codon.

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open readi...

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Which of the following statements about pseudogenes is true?


A) There often is little selective pressure to get rid of pseudogenes.
B) The sequences of a pseudogene and its functional counterpart must be very different.
C) In some gene families, pseudogenes outnumber functional genes.
D) Both a and b
E) Both a and c

F) B) and D)
G) A) and C)

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In DNA sequencing, ddNTPs are added at much lower concentrations than are dNTPs. If ddNTPs were added at higher concentrations, what would be the most likely result?


A) The ddNTPs would bind to one another.
B) All of the reactions would terminate early and long strands would not be produced.
C) The reactions would terminate early or late.
D) The ddNTPs would bind to DNA polymerase.
E) The ddNTPs would not bind to the dye.

F) A) and E)
G) A) and C)

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Nonfunctional copies of genes, known as _______, arise when mutations knock out the function of a gene.

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Which of the following statements about genomes is true?


A) Most genes found in the genomes of complex multicellular organisms are not required for survival.
B) As complexity increases from bacteria to yeast to invertebrate to vertebrate, gene number increases more than does the size of the genome.
C) As complexity increases from bacteria to yeast to invertebrate to vertebrate, the proportion of the genome consisting of coding sequence increases.
D) Both a and b
E) Both b and c

F) All of the above
G) C) and D)

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Gene inactivation studies have allowed us to


A) determine the minimal number of genes humans need to survive.
B) demonstrate that C. elegans needs most of its genes.
C) investigate the minimal number of genes needed to sustain life.
D) create artificial life in a test tube.
E) All of the above

F) A) and B)
G) C) and D)

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The diagram below shows high-throughput sequencing. At which step does PCR take place? The diagram below shows high-throughput sequencing. At which step does PCR take place?   A)  Step 1 B)  Step 2 C)  Step 3 D)  Step 4 E)  Step 5 or later


A) Step 1
B) Step 2
C) Step 3
D) Step 4
E) Step 5 or later

F) B) and C)
G) A) and E)

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Proteomics has been used to compare


A) DNA sequences between closely related species.
B) gene expression during embryonic development.
C) protein sequences between closely related species.
D) shotgun cloned sequences.
E) prokaryotic genomes.

F) B) and E)
G) B) and D)

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Which of the following statements comparing the worm C. elegans and the fly D. melanogaster is true?


A) The fly has a larger genome.
B) The fly has more genes.
C) The fly undergoes a more complicated developmental transformation.
D) Both a and b
E) Both a and c

F) A) and B)
G) B) and E)

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A comparison of the genomes of yeast and bacteria shows that only yeast has many genes for


A) energy metabolism.
B) cell wall synthesis.
C) intracellular protein targeting.
D) DNA-binding proteins.
E) RNA polymerase.

F) C) and D)
G) None of the above

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Which of the following statements about the human genome is false?


A) Over 50 percent of the genome contains transposons.
B) Almost every gene has introns.
C) Genes are evenly distributed over the genome.
D) About 2 percent of the genome codes for genes.
E) Humans have about the same number of genes as fruit flies have.

F) B) and C)
G) A) and B)

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You are told that a genome is about 10 Mb long, has 5,000 genes, and that 65% of its sequence is coding sequence. This genome most likely belongs to a(n)


A) virus.
B) bacterium.
C) yeast.
D) plant.
E) animal.

F) D) and E)
G) B) and E)

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Commonly known as the thale grass, _______, a member of the mustard family, is a model organism for a variety of studies in plant biology.

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The rRNA genes that encode for the various rRNAs are an example of _______ repetitive DNA.

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Which of the following statements about transposable elements is true?


A) Their insertion into genes can disrupt gene function.
B) Their position can vary in different strains of bacteria.
C) All are transposons.
D) Both a and b
E) Both b and c

F) A) and D)
G) D) and E)

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